$250.00
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CRM197 is a non-toxic mutant of diphtheria toxin having a single amino acid substitution of glutamic acid for glycine. CRM197 is a well-defined protein and functions as a carrier for polysaccharides and haptens making them immunogenic.
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Description
| Catalog Number | PeliCRM197® |
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Description
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PeliCRM197® diphtheria toxin mutant, recombinant. Research grade.
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Product Background
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PeliCRM197® is a non-toxic mutant of diphtheria toxin1,2 having a single amino acid substitution of glutamic acid for glycine. PeliCRM197® is a well-defined protein and functions as a carrier for polysaccharides and haptens making them immunogenic. It is utilized as a carrier protein in a number of approved conjugate vaccines for diseases such as meningitis and pneumococcal bacterial infections. PeliCRM197® is a recombinant form expressed in Pseudomonas fluorescens using the Pelican Expression Technology® platform. This product is available in both Research and cGMP grades. For more information on PeliCRM197® or our other vaccine carriers, please contact us or visit our knowledge center for articles, publications and other resources on PeliCRM197® and our other vaccine carriers. |
| Source | Pseudomonas fluorescens |
| Species | Corynebacterium diphtheriae |
| Storage | Store at 2-8 °C upon receipt |
| Purity | Purity: > 95% CRM197 by RP-HPLC
Endotoxin: < 100 EU/mg of protein Dimers and high molecular weight impurities: < 5% by SE-HPLC |
| Uses | Before use, aseptically reconstitute lyophilized PeliCRM197® with sterile deionized water to a concentration of 4 mg/mL CRM197. Reconstituted PeliCRM197® should be stored at 2-8 °C. |
Amino Acid Sequence
| GADDVVDSSK | SFVMENFSSY | HGTKPGYVDS |
| IQKGIQKPKS | GTQGNYDDDW | KEFYSTDNKY |
| DAAGYSVDNE | NPLSGKAGGV | VKVTYPGLTK |
| VLALKVDNAE | TIKKELGLSL | TEPLMEQVGT |
| EEFIKRFGDG | ASRVVLSLPF | AEGSSSVEYI |
| NNWEQAKALS | VELEINFETR | GKRGQDAMYE |
| YMAQACAGNR | VRRSVGSSLS | CINLDWDVIR |
| DKTKTKIESL | KEHGPIKNKM | SESPNKTVSE |
| EKAKQYLEEF | HQTALEHPEL | SELKTVTGTN |
| PVFAGANYAA | WAVNVAQVID | SETADNLEKT |
| TAALSILPGI | GSVMGIADGA | VHHNTEEIVA |
| QSIALSSLMV | AQAIPLVGEL | VDIGFAAYNF |
| VESIINLFQV | VHNSYNRPAY | SPGHKTQPFL |
| HDGYAVSWNT | VEDSIIRTGF | QGESGHDIKI |
| TAENTPLPIA | GVLLPTIPGK | LDVNKSKTHI |
| SVNGRKIRMR | CRAIDGDVTF | CRPKSPVYVG |
| NGVHANLHVA | FHRSSSEKIH | SNEISSDSIG |
| VLGYQKTVDH | TKVNSKLSLF | FEIKS |
Additional information
| Weight | 2 oz |
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| Dimensions | 3 × 3 × 1 in |
