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CRM197

$525.00 – $7,350.00

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CRM197 is a non-toxic mutant of diphtheria toxin having a single amino acid substitution of glutamic acid for glycine. CRM197 is a well-defined protein and functions as a carrier for polysaccharides and haptens making them immunogenic.

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Description

Catalog Number
CRM-197
Description
CRM197 diphtheria toxin mutant, recombinant. Pre-clinical grade.
Product Background

CRM197 is a non-toxic mutant of diphtheria toxin1,2 having a single amino acid substitution of glutamic acid for glycine. CRM197 is a well-defined protein and functions as a carrier for polysaccharides and haptens making them immunogenic. It is utilized as a carrier protein in a number of approved conjugate vaccines for diseases such as meningitis and pneumococcal bacterial infections. Pfenex CRM197 is a recombinant form expressed in Pseudomonas fluorescens using the Pfenex Expression TechnologyTM platform.

This product is available in both pre-clinical and cGMP grade.

For more information on CRM197 or our other vaccine carriers, please contact us or visit our knowledge center for articles, publications and other resources on CRM197 and our other vaccine carriers.
Source Pseudomonas fluorescens
Species Corynebacterium diphtheriae
Formulation CRM197 is lyophilized from a 0.2 micro-filtered solution containing 2 mg/mL CRM197, 50 mg/mL sucrose, 0.22 mg/mL sodium phosphate monobasic, 3 mg/mL sodium phosphate dibasic, 0.055 mg/mL polysorbate 80.

Product Molecular Mass: 58.4 kDa
Storage Store at 2-8 °C upon receipt
Purity  Purity: > 95% CRM197 by SDS-PAGE

A and B chain content < 5% of total CRM197

Endotoxin: < 100 EU/mg of protein by LAL method

Dimer: < 5%
Uses  Before use, aseptically reconstitute lyophilized CRM197 with sterile deionized water to a concentration of 4 mg/mL CRM197. Reconstituted CRM197 should be stored at 2-8 °C.

Amino Acid Sequence

GADDVVDSSK SFVMENFSSY HGTKPGYVDS
IQKGIQKPKS GTQGNYDDDW KEFYSTDNKY
DAAGYSVDNE NPLSGKAGGV VKVTYPGLTK
VLALKVDNAE TIKKELGLSL TEPLMEQVGT
EEFIKRFGDG ASRVVLSLPF AEGSSSVEYI
NNWEQAKALS VELEINFETR GKRGQDAMYE
YMAQACAGNR VRRSVGSSLS CINLDWDVIR
DKTKTKIESL KEHGPIKNKM SESPNKTVSE
EKAKQYLEEF HQTALEHPEL SELKTVTGTN
PVFAGANYAA WAVNVAQVID SETADNLEKT
TAALSILPGI GSVMGIADGA VHHNTEEIVA
QSIALSSLMV AQAIPLVGEL VDIGFAAYNF
VESIINLFQV VHNSYNRPAY SPGHKTQPFL
HDGYAVSWNT VEDSIIRTGF QGESGHDIKI
TAENTPLPIA GVLLPTIPGK LDVNKSKTHI
SVNGRKIRMR CRAIDGDVTF CRPKSPVYVG
NGVHANLHVA FHRSSSEKIH SNEISSDSIG
VLGYQKTVDH TKVNSKLSLF FEIKS

Additional information

Weight 2 oz
Dimensions 3 × 3 × 1 in