$525.00 – $7,350.00
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CRM197 is a non-toxic mutant of diphtheria toxin having a single amino acid substitution of glutamic acid for glycine. CRM197 is a well-defined protein and functions as a carrier for polysaccharides and haptens making them immunogenic.
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Description
| Catalog Number |
CRM-197
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Description
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CRM197 diphtheria toxin mutant, recombinant. Pre-clinical grade.
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Product Background
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CRM197 is a non-toxic mutant of diphtheria toxin1,2 having a single amino acid substitution of glutamic acid for glycine. CRM197 is a well-defined protein and functions as a carrier for polysaccharides and haptens making them immunogenic. It is utilized as a carrier protein in a number of approved conjugate vaccines for diseases such as meningitis and pneumococcal bacterial infections. Pfenex CRM197 is a recombinant form expressed in Pseudomonas fluorescens using the Pfenex Expression TechnologyTM platform. This product is available in both pre-clinical and cGMP grade. For more information on CRM197 or our other vaccine carriers, please contact us or visit our knowledge center for articles, publications and other resources on CRM197 and our other vaccine carriers. |
| Source | Pseudomonas fluorescens |
| Species | Corynebacterium diphtheriae |
| Formulation | CRM197 is lyophilized from a 0.2 micro-filtered solution containing 4 mg/mL CRM197, 50 mg/mL sucrose, 0.22 mg/mL sodium phosphate monobasic, 3 mg/mL sodium phosphate dibasic, 0.055 mg/mL polysorbate 80.
Product Molecular Mass: 58.4 kDa |
| Storage | Store at 2-8 °C upon receipt |
| Purity | Purity: > 95% CRM197 by SDS-PAGE
A and B chain content < 5% of total CRM197 Endotoxin: < 100 EU/mg of protein by LAL method Dimer: < 5% |
| Uses | Before use, aseptically reconstitute lyophilized CRM197 with sterile deionized water to a concentration of 4 mg/mL CRM197. Reconstituted CRM197 should be stored at 2-8 °C. |
Amino Acid Sequence
| GADDVVDSSK | SFVMENFSSY | HGTKPGYVDS |
| IQKGIQKPKS | GTQGNYDDDW | KEFYSTDNKY |
| DAAGYSVDNE | NPLSGKAGGV | VKVTYPGLTK |
| VLALKVDNAE | TIKKELGLSL | TEPLMEQVGT |
| EEFIKRFGDG | ASRVVLSLPF | AEGSSSVEYI |
| NNWEQAKALS | VELEINFETR | GKRGQDAMYE |
| YMAQACAGNR | VRRSVGSSLS | CINLDWDVIR |
| DKTKTKIESL | KEHGPIKNKM | SESPNKTVSE |
| EKAKQYLEEF | HQTALEHPEL | SELKTVTGTN |
| PVFAGANYAA | WAVNVAQVID | SETADNLEKT |
| TAALSILPGI | GSVMGIADGA | VHHNTEEIVA |
| QSIALSSLMV | AQAIPLVGEL | VDIGFAAYNF |
| VESIINLFQV | VHNSYNRPAY | SPGHKTQPFL |
| HDGYAVSWNT | VEDSIIRTGF | QGESGHDIKI |
| TAENTPLPIA | GVLLPTIPGK | LDVNKSKTHI |
| SVNGRKIRMR | CRAIDGDVTF | CRPKSPVYVG |
| NGVHANLHVA | FHRSSSEKIH | SNEISSDSIG |
| VLGYQKTVDH | TKVNSKLSLF | FEIKS |
Additional information
| Weight | 2 oz |
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| Dimensions | 3 × 3 × 1 in |